沈立新

点击次数:221 发布时间:2024-03-28

沈立新
教授,博士生导师,生物技术与工程硕士专业学位学科负责人。
通信地址 西北大学生命科学学院,西安市太白北路 229 号,邮编 710069
Tel: 029-88302132(实验室)Cell phone:13991902992; E-mail : shenlx@nwu.edu.cn
主要研究方向: 病原微生物分子致病、耐药机制及其新型药物研究;利用分子生物学及微生物工程技术进
行生物活性物质生产及性质研究;微生物在医用材料、废物处理、农药方面的应用。
教育及工作经历 :
西北大学化工学院 化学工程专业,学士学位;
西北大学化学工程系 生物化工专业,硕士学位;
西北大学化学工程学院生物化工系,讲师;
华东理工大学生物工程学院生物工程专业,博士学位,导师:魏东芝;)
西北大学生命科学学院生物技术系,副教授;
维也纳大学生物中心,访问学者,导师:Emmanuelle Charpentier)
西北大学生命科学学院生物技术系,教授;
学术兼职 :
中国微生物学会委员;中国医学微生物学与免疫学专业委员会委员;陕西省微生物学会理事;中国医药
生物技术协会生物安全专业委员会委员 陕西省医学微生物委员会委员;
科研项目 :
1. “基于铜绿假单胞菌致病性的中药抗致病活性成分筛选及作用机理研究”,2018 年陕西省重点研发
2. “铜绿假单胞菌中碳青霉烯类抗生素的耐药机制研究”,2011 年国家自然科学基金(81171620);
3. “铜绿假单胞菌降解多环芳烃关键基因的筛选和研究”,2011 年陕西省教育厅(11JK0611);
4. “MuxABC-OpmB 双向影响铜绿假单胞菌耐药性的分子机制研究”,2011 年陕西省科技厅(2011JM  
4013);
4. “GAS 中高通量全基因组基因表达系统的研究”,2007 年省教委攻关项目;
6.“多环芳烃生物降解的研究”,2004 年陕西省科技厅攻关项目;
7.“生物手段降解多环芳烃的研究”,2004 年西安市科委攻关项目;
8.“双株重组大肠杆菌催化合成谷胱甘肽的研究”,2002 省教委攻关项目。
发表文章 :
1. Songzhe Fu*, Yixiang Zhang, Rui Wang, Zhiqiang Deng, Fenglan He, Xiaotong Jiang, Lixin Shen* .  
Longitudinal wastewater surveillance of four key pathogens during an unprecedented large-scale COVID-19  
outbreak in China facilitated a novel strategy for addressing public health priorities–A proof of concept study.
Water Research. 2023. Oct.17. doi: 10.1016/j.watres.2023.120751.
2. Songzhe Fu*, Yixiang Zhang, Rui Wang, Zhiguang Qiu, Weizhi Song, Qian Yang, Lixin Shen*. A novel  
culture-enriched metagenomic sequencing strategy effectively guarantee the microbial safety of drinking water  
by uncovering the low abundance pathogens. Journal of Environmental Management. 2023 Jul.31. doi:
10.1016/j.jenvman.2023.118737.
3. Na Li, Wan Liu, Xiaoyan Zheng*, Qing Wang, Lixin Shen * , Junfeng Hui, Daidi Fan*. Antimicrobial Hydrogel  
with Multiple pH-ResponsivenessforInfected Burn Wound Healing. Nano Research . 2023 Jun. 22;
11139−11148. doi 10.1007/s12274-023-5751-6.  
4. Qingqing Sun, Lixin Shen* , Bai-Ling Zhang, Jiaoyang Yu, Fu Wei, Yanmei Sun, Wei Chen, Shiwei Wang.  
Advance on Engineering of Bacteriophages by Synthetic Biology. Infection and Drug Resistance .2023  
May.31;16:1941-1953. doi: 10.2147/IDR.S402962.
5. Ning Mao, Shuwen Xue, Guangming Zhang, Sizhe Xia, Xiaohui Chen, Wencai You, Yanmei Sun, Lixin Shen
*, Shiwei Wang*. Crude oil degradation in oilfield produced water by immobilized microbes and oil recovery  
improvement after its reinjection. International Biodeterioration & Biodegradation . 2022 Sep 173: 105452
6. Xiaofeng Han, Mengyue Nan, Xinyu Cai, Boling Qiao, Lin Chen and Lixin Shen*. Sennoside A inhibits quorum  
sensing system to attenuate its regulated virulence and pathogenicity via targeting LasR in Pseudomonas  
aeruginosa. Frontiers in Microbiology .2022 Nov 03: 13:1042214. doi: 10.3389/fmicb.2022.1042214.  
eCollection 2022.
7. Yongheng Yang, Mengyun Li, Yongtao Ma, Wei Ye, Yue Si, Xuyang Zheng, He Liu, Linfeng Cheng, Liang  
Zhang, Hui Zhang, Xijing Zhang, Yingfeng Lei, Lixin Shen *, Fanglin Zhang* Hongwei Ma*. LncRNA NEAT1  
Potentiates SREBP2 Activity to Promote Inflammatory Macrophage Activation and Limit Hantaan Virus  
Propagation. Frontiers in Microbiology . 2022 Apr 13; 13:849020. doi: 0.3389/fmicb. 2022.  
849020. eCollection  
8. Jiali Yang, Mengyuan Li, Mingcheng Yuan, Peiyu Bian, Yangchao Dong, Haijun Zhang, Huanyu Luo, Zhifeng  
Xue, Yuan Wang, Fanglin Zhang, Lixin Shen *, Yingfeng Lei*. Axl /÷ neurons promote JEV infection by  
dampening the innate immunity. Virus Res .2022 Jan 2; 307:198605. doi: 0.1016/j.virusres.2021.198605. Epub  
2021 Oct 16.  
9. Shiwei Wang, Yuqi Feng, Xiaofeng Han, Xinyu Cai, Liu Yang, Chaolan Liu and Lixin Shen . Inhibition of  
Virulence Factors and Biofifilm Formation by Wogonin Attenuates Pathogenicity of Pseudomonas aeruginosa  
PAO1 via Targeting pqs Quorum-Sensing System. Int. J. Mol. Sci . 2021, 22, 12699.  
doi.org/10.3390/ijms222312699 10. Lixin Shen , Lang Gao, Mengjiao Yang, Jian Zhang, Yulu Wang, Yuqi Feng, Liping Wang Shiwei Wang.  
Deletion of the PA4427-PA4431 Operon of Pseudomonas aeruginosa PAO1 Increased Antibiotics Resistance  
and Reduced Virulence and Pathogenicity by Affecting Quorum Sensing and Iron Uptake. Microorganisms .  
2021 May 14;9(5):1065. doi: 10.3390/microorganisms9051065.  
11. Huanhuan Ning, Wei Zhang, Jian Kang, Tianbing Ding, Xuan Liang, Yanzhi Lu, Chengxuan Guo, Wenjie
Sun, Huapeng Wang, Yinlan Bai, Lixin Shen *. Subunit vaccine ESAT-6:c-di-AMP delivered by intranasal  
route elicits immune responses and protects against Mycobacterium tuberculosis infection. Front Cell Infect  
Microbiol . 2021 Mar 22; 11:647220. doi: 10.3389/fcimb.2021.647220.  
12. Pansong Zhang Qiao Guo Zhihua Wei Qin Yang Zisheng Guo Lixin Shen Kangmin Duan Lin Chen.  
Baicalin Represses Type Three Secretion System of Pseudomonas aeruginosa through PQS System. Molecules  
2021, 26(6), 1497; https://doi.org/10.3390/molecules26061497
13. Shiwei Wang, Qiuqiu Hou, Qianqian Guo, Jian Zhang, Yanmei Sun, Hong Wei, Lixin Shen *. Isolation and  
Characterization of a Deoxynivalenol-Degrading Bacterium Bacillus licheniformis YB9 with the Capability of  
Modulating Intestinal Microbial Flora of Mice. Toxins (Basel). 2020 Mar; 12(3): 184-197. doi:  
10.3390/toxins12030184.
14. Yulu Wang, Liyue Wang, Jian Zhang, Xintong Duan, Yuqi Feng, Shiwei Wang, Lixin Shen *. PA0335, a Gene  
Encoding Histidinol Phosphate Phosphatase, Mediates Histidine Auxotrophy in Pseudomonas aeruginosa. Appl  
Environ Microbiol . 2020 Mar; 86(5): e02593-19.
15. Kerong Wang, Hongwei Ma, He Liu, Wei Ye, Zhuo Li, Linfeng Cheng, Liang Zhang, Yingfeng Lei, Lixin  
Shen *, Fanglin Zhang*. The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy  
Flux to Restrain Host Innate Immune Responses. Cell Rep . 2019 May 14; 27(7):2075-2091.e5. doi:  
10.1016/j.celrep.2019.04.061.
16. Huanhuan Ning, Lifei Wang, Jie Zhou, Yanzhi Lu, Jian Kang, Tianbing Ding, Lixin Shen , Zhikai Xu, Yinlan  
Bai. Recombinant BCG With Bacterial Signaling Molecule Cyclic di-AMP as Endogenous Adjuvant Induces  
Elevated Immune Responses After Mycobacterium tuberculosis Infection. Front Immunol . 2019; 10: 1519.  
doi: 10.3389/fimmu.2019.01519.  
17. Rong Yan, Shikun Hu, Ning Ma, Peiqing Song, Qingqing Liang, Huiqun Zhang, Yanqi Li, Lixin  
Shen , Kangmin Duan, Lin Chen. Regulatory Effect of DNA Topoisomerase I on T3SS Activity, Antibiotic  
Susceptibility and Quorum- Sensing-Independent Pyocyanin Synthesis in Pseudomonas aeruginosa. Int J Mol  
Sci . 2019 Mar 5;20(5):1116. doi: 10.3390/ijms20051116.
18. Yue Wang, Jian Zhang, Yulu Wang, Kerong Wang, Hong Wei, Lixin Shen *. Isolation and characterization of  
the Bacillus cereus BC7 strain, which is capable of zearalenone removal and intestinal flora modulation in mice.  
Toxicon . 2018, 1;155:9-20. Epub 2018 Sep 26.
19. Lang Gao, Zisheng Guo, Yue Wang, Yulu Wang, Kerong Wang, Bo Li, Lixin Shen *. The two-operon-coded  
ABC transporter complex FpvWXYZCDEF is required for Pseudomonas aeruginosa growth and virulence under  
iron-limiting conditions. J Membr Biol. 2018, 251(1): 91-104. doi: 10.1007/s00232-017-9979-5. Epub 2017  
Oct 3
20. Bo Fu, Qiaolian Wu, Minyan Dang, Dangdang Bai, Qiao Guo, Shen Lixin *, Duan Kangmin*. Inhibition  
of Pseudomonas aeruginosa biofilm formation by traditional Chinese-medicinal herb herba patriniae. BioMed  
Research International . 2017, 136: 1-10.
21. Ying Liang, Zisheng Guo, Lang Gao, Qianqian Guo, Liyue Wang, Kangmin Duan*, Lixin Shen *. The role of  
the temperature-regulated acyltransferase (PA3242) on growth, antibiotic resistance and virulence in  
Pseudomonas aeruginosa. Microbial Pathogenesis . 2016, 101 :126-135.
22. Bobo Wang, Bo Li, Ying Liang, Jing Li, Lang Gao, Lin Chen, Kangmin Duan *, Lixin Shen *. Pleiotropic effects  
of temperature-regulated 2-OH-lauroytransferase (PA0011) on Pseudomonas aeruginosa antibiotic resistance,  
virulence and type III secretion system. Microbial Pathogenesis . 2016, 91:5-17. DOI:  
doi.org/10.1016/j.micpath.2015.11.003
23. Jing Qi, Jing Li, Bobo Wang, Huanhuan Ning, Yingjuan Wang, Weina Kong, Lixin Shen *. Genetic  
Determinants Involved in the Biodegradation of Naphthalene and Phenanthrene in Pseudomonas aeruginosa
PAO1. Environ Sci Pollut Res . 2015, 22:6743–6755.  
24. Bo Li, Bobo Wang, Xiangli Gao, Lang Gao, Ying Liang, Kangmin Duan*, Lixin Shen *. The Zinc Uptake  
System (znuA Locus) is Important for Bacterial Adhesion and Virulence in Pseudomonas aeruginosa . 2014,
ICBEEE.
25. Yang L, Chen L, Shen L , Duan K. Construction of a tightly-controlled expression system for use in  
Pseudomonas . Biotechnol Lett . 2013 Nov;35(11):1901-6. doi: 10.1007/s10529-013-1280-9. Epub 2013 Jul 24.  
PMID: 23881319.
26. Kong W, Chen L, Zhao J, Shen T, Surette MG, Shen L , Duan K. Hybrid sensor kinase PA1611 in Pseudomonas  
aeruginosa regulates transitions between acute and chronic infection through direct interaction with RetS. Mol  
Microbiol . 2013 May;88(4):784-97. doi: 10.1111/mmi.12223. Epub 2013 Apr 21. PMID: 23560772.
27. Lixin Shen , Xiangli Gao, Jinhua Wei, Lin Chen, Xin Zhao, Bo Li, Kangmin Duan. PA2800 Plays an Important  
Role in Both Antibiotic Susceptibility and Virulence in Pseudomonas aeruginosa . Curr Microbiol , 2012  
Nov;65(5):601-9. Epub 2012 Aug 10.
28. Lixin Shen , Yanling Ma, Haihua Liang. Characterization of a novel gene related to antibiotic susceptibility in  
Pseudomonas aeruginosa , The Journal of Antibiotics , 2012, 65: 59–65.
29. Yang L, Chen L, Shen L , Surette M, Duan K. Inactivation of MuxABC-OpmB transporter system in  
Pseudomonas aeruginosa leads to increased ampicillin and carbenicillin resistance and decreased virulence. J  
Microbiol. 2011 Feb; 49(1):107-14. doi: 10.1007/s12275-011-0186-2. Epub 2011 Mar 3. PMID: 21369987. 30. Lingfang Yin, Huiping Sun, Dan sun, Sujuan Zhang, Lixin Shen . Evaluation of Rif-resiatance as the screening  
indicator for Multi-drug resistant tuberculosis. IEEE 2011
31. Lixin Shen , Dan Zhang, Ying Shi, Haihua Liang, Kangmin Duan. Genomic investigation of the role of  
antibiotics as signals molecules in Pseudomonas aeruginosa . ICBBE 2009(EI).
32. Haihua Liang, Lingling Li, Weina Kong, Lixin Shen , Kangmin Duan. Identification of a novel regulator of the  
quorum-sensing systems in Pseudomonas aeruginosa . FEMS Microbiol Lett., 2009, 293(2):196-204 .
33. Shen L ., Y. Shi., D. Zhang, J. Wei, M. G. Surette, and K. Duan. Modulation of secreted virulence factor genes  
by subinhibitory concentrations of antibiotics in Pseudomonas aeruginosa . J. Microbiol . 2008 46:441-447  
专利: 一种用于抗菌药物筛选的新载体, 沈立新等 . CN2015176236.5
番泻苷 A 在制备抗铜绿假单胞菌感染的制剂中的用途,沈立新,韩味枫等. CN114246876A  
著作 《重组蛋白的纯化及分析》. 化学工业出版社出版, 2004, 4 北京, 范代娣, 沈立新 ,米钰
获奖 铜绿假单胞菌致病性及耐药性机制研究,陕西省高等学校科学技术一等奖,2011(第二)

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